Lettuce variety NUN 06193 LTL

ABSTRACT

The disclosure provides a new and distinct lettuce variety NUN 06193 LTL, as well as seeds and plants and heads or leaves thereof. NUN 06193 LTL is a green long-day Romaine lettuce of the open type and with solid ribs, comprising resistance to Downy Mildew (Bremia lactucae) isolates B1:16-28 and 31-32 EU, Lettuce Mosaic Virus (LMV), and Rhizomonas subefaciens Strain Ls 1 (Corky Root).

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Application No.62/676,626, filed May 25, 2018, which is hereby incorporated byreference in its entirety.

FIELD OF THE DISCLOSURE

The disclosure relates to the field of plant breeding and, morespecifically, to lettuce variety NUN 06193 LTL. The disclosure furtherrelates to vegetative reproductions of NUN 06193 LTL, methods for tissueculture of NUN 06193 LTL and regenerating a plant from such a tissueculture, and to phenotypic variants of NUN 06193 LTL.

BACKGROUND OF THE DISCLOSURE

The goal of plant breeding is to combine various desirable traits in asingle variety. Such desirable traits may include greater yield,resistance to diseases, insects or other pests, tolerance to heat anddrought, better agronomic quality, higher nutritional value, enhancedgrowth rate and improved shelf life.

The development of commercial lettuce cultivars or varieties requiresthe crossing of lettuce plants, and the evaluation of the crosses.Pedigree breeding and recurrent selection are examples of breedingmethods that are used to develop cultivars from breeding populations.Breeding programs combine desirable traits from two or more varieties orvarious broad-based sources into breeding pools from which cultivars aredeveloped by selfing and selection of desired phenotypes. The newcultivars are crossed with other varieties and the inbred lines orhybrids from these crosses are evaluated to determine which havecommercial potential.

All cultivated forms of lettuce belong to the highly polymorphic speciesLactuca sativa that is grown for its edible head and leaves. Lactucasativa is in the Asteraceae (Compositae) family. Lettuce is related tochicory, sunflower, aster, dandelion, artichoke and chrysanthemum. L.sativa is one of about 300 species in the genus Lactuca. There are manytypes of lettuce, and new types are constantly in development. Types oflettuce include Cutting/Leaf, Iceberg/Crisphead, Cos or Romaine,Batavian, Salinas Group, Latin, Butterhead, Great Lakes Group, Eastern(Ithaca) Group, Bibb, Vanguard Group, Multileaf or Stem lettuce. Lettuceis consumed nearly exclusively as fresh, raw product and occasionally asa cooked vegetable. It is popularly used in salads, wraps, andsandwiches.

Fresh lettuce is available in the United States year-round although thegreatest supply is from May through October. For planting purposes, thelettuce season is typically divided into three categories: early, midand late, with the coastal areas planting from January to August, andthe desert regions planting from August to December. California andArizona are the two largest producers of lettuce in the United States.

Changes in lifestyles and the demand from restaurants and catering firmsfor colorful and interesting garnish for sandwiches and ready-to-useprocessed salads continue to rise. As a result, there is a demand forbreeding companies to develop new varieties with specific shapes ofleaves, specific average size of leaves, glossiness, prominent color,taste, and a wide variety of texture. Other breeding objectives includedisease or pest resistance, yield, prolonged shelf life and suitabilityto climatic requirements.

SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure provides for lettuce variety NUN 06193 LTL, productsthereof, and methods of using the same. NUN 06193 LTL is a greenlong-day Romaine lettuce of the open type, with solid ribs.

In one aspect, the disclosure provides a seed of lettuce variety NUN06193 LTL, wherein a representative sample of said seed will bedeposited under Accession Number NCIMB 43665. The disclosure alsoprovides for a plurality of seeds of lettuce variety NUN 06193 LTL. Thelettuce seed of variety NUN 06193 LTL may be provided as an essentiallyhomogeneous population of lettuce seed. The population of seed oflettuce variety NUN 06193 LTL may be particularly defined as essentiallyfree from other seed. The seed population may be grown into plants toprovide an essentially homogeneous population of lettuce plants asdescribed herein.

The disclosure also provides for a plant grown from a seed of lettucevariety NUN 06193 LTL and a plant part thereof. In another aspect, thedisclosure provides for an inbred variety of NUN 06193 LTL. Thedisclosure also provides for a progeny of lettuce variety NUN 06193 LTL.In another aspect, the disclosure provides a plant or a progenyretaining all or all but one, two or three of the “distinguishingcharacteristics” of lettuce variety NUN 06193 LTL, and methods ofproducing that plant or progeny.

In another aspect, the disclosure provides a plant or a progeny havingall the physiological and morphological characteristics of variety NUN06193 LTL when grown under the same environmental conditions. In anotheraspect, the plant or progeny has all or all but one, two or three of thephysiological characteristics of lettuce variety NUN 06193 LTL whenmeasured under the same environmental conditions and e.g., evaluated atsignificance levels of 1%, 5% or 10% significance (which can beexpressed as a p-value) for quantitative characteristics, wherein arepresentative sample of seed of variety NUN 06193 LTL will be depositedunder Accession Number NCIMB 43665. In another aspect, the plant orprogeny has all or all but one, two or three of the physiological andmorphological characteristics as listed in Tables 1, 2, and 3 of lettucevariety NUN 06193 LTL when measured under the same environmentalconditions and e.g., evaluated at significance levels of 1%, 5% or 10%significance (which can also be expressed as a p-value) for quantitativecharacteristics.

In another aspect, a plant of lettuce variety NUN 06193 LTL or a progenythereof has 10, 11, or more or all of the following distinguishingcharacteristics when compared to Reference Variety NUN 06109 LTL(Copious) as shown in Table 4: 1) taller plant height; 2) narrowerspread of frame leaves; 3) narrower width of mature leaf; 4) longerlength/width index of mature leaf; 5) an entire indentation of matureleaf; 6) absence of undulations of the apical margin in the mature leaf;7) smaller size of mature leaf; 8) duller mature leaf; 9) absence ofblistering in the mature leaf; 10) thinner mature leaf; and 11) smallersize of plant head.

In another aspect, a plant of lettuce variety NUN 06193 LTL or a partthereof comprises resistance to Downy Mildew (Bremia lactucae) isolatesB1:16-28 and 31-32 EU, Lettuce Mosaic Virus (LMV) Strain Ls 1, andRhizomonas subefaciens (Corky Root), measured according to UPOVstandards described in TG/13/11.

In another aspect, the disclosure provides for a plant part obtainedfrom variety NUN 06193 LTL, wherein said plant part is: a leaf, a partof a leaf, a head, a part of a head, a fruit, a part of a fruit, pollen,an ovule, a cell, a petiole, a shoot or a part thereof, a stem or a partthereof, a root or a part thereof, a root tip, a cutting, a seed, a partof a seed, seed coat or another maternal tissue which is part of a seedgrown on said variety, hypocotyl, cotyledon, a pistil, an anther, or aflower or a part thereof. Heads and leaves are particularly importantplant parts. In another aspect, the plant part obtained from variety NUN06193 LTL is a cell, optionally a cell in a cell or tissue culture. Thatcell may be grown into a plant of lettuce variety NUN 06193 LTL.

The disclosure also provides a cell culture of lettuce variety NUN 06193LTL and a plant regenerated from lettuce variety NUN 06193 LTL, whereinthe plant has all the characteristics of lettuce variety NUN 06193 LTLwhen grown under the same environmental conditions, as well as methodsfor culturing and regenerating lettuce variety NUN 06193 LTL.Alternatively, a regenerated plant may have one characteristic that isdifferent from lettuce variety NUN 06193 LTL.

The disclosure further provides a vegetatively propagated plant ofvariety NUN 06193 LTL having all or all but one, two or three of themorphological and physiological characteristics lettuce variety NUN06193 LTL when grown under the same environmental conditions.

The disclosure also provides a lettuce head and/or a lettuce leafproduced on a plant grown from a seed of lettuce variety NUN 06193 LTL.

In another aspect, the disclosure provides a seed growing or grown on aplant of lettuce variety NUN 06193 LTL (e.g., produced after pollinationof the flower of lettuce variety NUN 06193 LTL). The disclosure alsoprovides for an F1 progeny of lettuce variety NUN 06193 LTL.

Definitions

“Lettuce” refers herein to plants of the species Lactuca sativa L. Themost commonly eaten parts of a lettuce plant are the head or a leaf. Thehead comprises a core and leaves, which may be divided in inner andouter leaves.

“Cultivated lettuce” refers to plants of Lactuca sativa (e.g.,varieties, breeding lines or cultivars of the species L. sativa as wellas crossbreds thereof, or crossbreds with other Lactuca sativa species,or even with other Lactuca species), cultivated by humans and havinggood agronomic characteristics.

“Romaine lettuce” or “cos lettuce” refers to a type of lettuce with atall head comprising leaves with firm ribs. The leaves are oftenrelatively dark.

The terms “lettuce plant designated NUN 06193 LT”, “NUN 06193 LT”,“inbred NUN 06193”, “06193 LT” or “lettuce 06193” are usedinterchangeably herein and refer to a lettuce plant of variety NUN 06193LTL, representative seed of which will be deposited under AccessionNumber NCIMB 43665.

A “seed of NUN 06193 LTL” refers to a lettuce seed which can be growninto a plant of lettuce variety NUN 06193 LTL, wherein a representativesample of viable seeds of lettuce variety NUN 06193 LTL will bedeposited under Accession Number NCIMB 43665. A seed can be in any stageof maturity, for example, a mature, viable seed, or an immature,non-viable seed. A seed comprises an embryo and maternal tissues.

An “embryo of NUN 06193 LTL” refers to an embryo as present in a seed oflettuce variety NUN 06193 LTL, a representative sample of seed oflettuce variety NUN 06193 LTL will be deposited under Accession NumberNCIMB 43665.

A “seed grown on NUN 06193 LTL” refers to a seed grown on a mature plantof lettuce variety NUN 06193 LTL or inside a fruit of lettuce varietyNUN 06193 LTL. The “seed grown on NUN 06193 LTL” contains tissues andDNA of the maternal parent, lettuce variety NUN 06193 LTL. The “seedgrown on NUN 06193 LTL” contains an F1 embryo. When said seed isplanted, it grows into a first-generation progeny plant of lettucevariety NUN 06193 LTL. Since NUN 06193 LTL is an inbred variety and thushighly homozygous, the set of chromosomes inherited by thefirst-generation progeny is predictable.

An “essentially homogeneous population of lettuce seed” is a populationof seeds where at least 97%, 98%, 99% or more of the total population ofseed are seed of lettuce variety NUN 06193 LTL.

An “essentially homogeneous population of lettuce plants” is apopulation of plants where at least 97%, 98%, 99% or more of the totalpopulation of plants are plants of lettuce variety NUN 06193 LTL.

The phrase “essentially free from other seed” refers to a population ofseed where less than 3%, 2%, 1%, or even less, of the total populationof seed is seed that is not a lettuce seed or, in another option, lessthan 3%, 2%, 1%, or less, of the total population of seed is seed thatis not seed of lettuce variety NUN 06193 LTL.

“Tissue culture” or “cell culture” refers to a composition comprisingisolated cells of the same or a different type or a collection of suchcells organized into parts of a plant. Tissue culture of various tissuesof lettuce and regeneration of plants therefrom is well known and widelypublished (see e.g., Teng et al., HortScience. 1992, 27(9): 1030-1032;Teng et al., HortScience. 1993, 28(6): 669-1671; Zhang et al., Journalof Genetics and Breeding. 1992, 46(3): 287-290). Similarly, methods ofpreparing cell cultures are known in the art.

“USDA descriptors” are the plant variety descriptors described forlettuce in the “Objective description of Variety—Lettuce (Lactuca sativaL.)”, as published by U.S. Department of Agriculture, AgriculturalMarketing Service, Science and Technology, Plant Variety ProtectionOffice, Beltsville, Md. 20705 and which can be downloaded from theworld-wide web at ams.usda.gov/underservices/plant-variety-protection/pvpo-c-forms under lettuce. “Non-USDAdescriptors” are other descriptors suitable for describing lettuce.

“UPOV descriptors” are the plant variety descriptors described forlettuce in the “Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability”, TG/13/11 (Geneva 2006, last updated2017-04-05), as published by UPOV (International Union for theProtection of New Varieties and Plants), and which can be downloadedfrom the world-wide web at upov.int/under edocs/tgdocs/en/tg013.pdf,which is hereby incorporated by reference in its entirety. Likewise,“UPOV methods” to determine specific parameters for the characterizationof lettuce are described at upov.int.

“RHS” or “RHS color” refers to the color chart of the RoyalHorticultural Society (UK), which publishes a botanical color chartquantitatively identifying colors by a defined numbering system. Thechart may be purchased from Royal Horticulture Society Enterprise LtdRHS Garden; Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS colorchart 2007.

“Plant part” includes any part of a plant, such as a plant organ (e.g.,harvested or non-harvested fruits), a plant cell, a plant protoplast, aplant cell tissue culture or a tissue culture from which a whole plantcan be regenerated, a plant cell that is intact in a plant, a clone, amicropropagation, plant callus, a plant cell clump, a plant transplant,a vegetative propagation, a seedling, a fruit, a harvested fruit, a partof a fruit, a leaf, a part of a leaf, pollen, an ovule, an embryo, apetiole, a shoot or a part thereof, a stem or a part thereof, a root ora part thereof, a root tip, a cutting, a seed, a part of a seed,hypocotyl, cotyledon, a scion, a graft, a stock, a rootstock, a pistil,an anther, and a flower or parts of any of these and the like. Seed canbe mature or immature. Pollen or ovules may be viable or non-viable.Also, any developmental stage is included, such as seedlings, cuttingsprior or after rooting, mature plants or leaves. Alternatively, a plantpart may also include a plant seed which comprises one or two sets ofchromosomes derived from the parent plant (e.g., from lettuce varietyNUN 06193 LTL). An F1 progeny produced from self-pollination of theinbred variety NUN 06193 LTL will thus comprise two sets of chromosomesderived from lettuce variety NUN 06193 LTL while an F1 progeny derivedfrom cross-fertilization of lettuce variety NUN 06193 LTL will compriseonly one set of chromosomes from lettuce variety NUN 06193 LTL and theother set of chromosomes from the other parent.

“Reference variety” refers herein to variety NUN 06109 LTL, a varietyfrom Nunhems B.V. with commercial name Copious, which has been plantedin a trial together with NUN 06193 LTL. USDA and Non-USDA descriptors oflettuce variety NUN 06193 LTL were compared to the USDA descriptors ofvariety NUN 06109 LTL as shown in Tables 1 and 2. The distinguishingcharacteristics between lettuce variety NUN 06193 LTL and the ReferenceVariety (NUN 06109 LTL) are shown in Table 4.

“Head” as used herein refers to lettuce heads, i.e., the plant withoutthe root system, for example, substantially all harvested leaves.Encompassed are immature leaves (e.g., “baby leaf”) and mature leaves.

The “base” of a plant is the part of a lettuce plant where the leavesare attached to the root system of the plant.

“Core length” of the internal lettuce stem is measured from the base ofthe cut and trimmed head to the tip of the stem.

“Head weight” refers to the mean weight of saleable lettuce head, cutand trimmed to market specifications.

“Head diameter” refers to the mean diameter of the cut and trimmed head,sliced vertically, and measured at the widest point perpendicular to thestem.

“Head height” refers to the mean height of the cut and trimmed head,sliced vertically, and measured from the base of the cut stem to theleaf tip.

“Core Length to Head Diameter Ratio (CLHD Ratio)” refers to the meancore length/head diameter ratio. It is calculated by dividing the meancore length with the mean head diameter. This is an indication of thehead shape and of the ability of a lettuce plant to reduce the amount ofsurface which is on or close to the ground.

“Harvested plant material” refers herein to plant parts (e.g., leaves orheads detached from the whole plant) which have been collected forfurther storage and/or further use.

“Yield” means the total weight of all lettuce heads or leaves harvestedper hectare of a particular line or variety. It is understood that“yield” expressed as weight of all lettuce heads or leaves harvested perhectare can be obtained by multiplying the number of plants per hectaretimes the “yield per plant”.

“Marketable yield” means the total weight of all marketable lettuceheads or leaves harvested per hectare of a particular line or variety,e.g., lettuce heads or leaves suitable for being sold for freshconsumption, having good color, glossiness, size and texture and no orvery low levels of deficiencies. A “marketable lettuce head or leaf” isa head or leaf that has commercial value.

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant showing the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions, preferably in the same experiment;the referred-to-plant can be a plant from which it was derived, e.g.,the progeny plant, the progenitor plant, the parent, the recurrentparent, the plant used for tissue- or cell culture, etc. A physiologicalor morphological characteristic can be a numerical characteristic or anon-numerical characteristic. In one aspect, a plant has “all but one,two or three of the physiological and morphological characteristics” ofa referred-to-plant, or “all the physiological and morphologicalcharacteristics” of Tables 1, 2, and 3 or “all or all but one, two orthree of the physiological and morphological characteristics” of Tables1, 2, and 3.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5% or 10% if theyare numerical (quantitative), or for having an identical degree (ortype) if not numerical (not quantitative), if measured under the sameenvironmental conditions. For example, a progeny plant or a Single LocusConverted plant or a mutated plant of lettuce variety NUN 06193 LTL mayhave one or more (or all) of the essential physiological and/ormorphological characteristics of said variety listed in Tables 1, 2, and3, as determined at the 5% significance level (i.e., p<0.05), when grownunder the same environmental conditions.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein to the characteristicswhich distinguish a new variety from other lettuce varieties, such asthe Reference Variety (i.e., are different), when grown under the sameenvironmental conditions. The distinguishing characteristics betweenlettuce variety NUN 06193 LTL, and Reference Variety (NUN 06109 LTL) aredescribed in Table 4. When comparing lettuce variety NUN 06193 LTL toother varieties, the distinguishing characteristics may be different. Inone aspect, the distinguishing characteristics may therefore include atleast one, two, three or more (or all) of the characteristics listed inTables 1, 2, and 3. All numerical distinguishing characteristics arestatistically significantly different at p<0.05 between lettuce varietyNUN 06193 LTL and the other variety (e.g., the Reference Variety).

Lettuce Variety NUN 06193 LTL has the following distinguishingcharacteristics when compared to Reference Variety NUN 06109 LTL asshown in Table 4: : 1) taller plant height; 2) narrower spread of frameleaves; 3) narrower width of mature leaf; 4) longer length/width indexof mature leaf; 5) an entire indentation of mature leaf; 6) absence ofundulations of the apical margin in the mature leaf; 7) smaller size ofmature leaf; 8) duller mature leaf; 9) absence of blistering in themature leaf; 10) thinner mature leaf; and 11) smaller size of planthead, where the characteristics of lettuce variety NUN 06193 LTL arecompared to the characteristics of the Reference Variety (NUN 06109LTL), when grown under the same environmental conditions.

Thus, a lettuce plant “comprising the distinguishing characteristics ofNUN 06193 LTL” (such as a progeny plant) refers herein to a plant whichdoes not differ significantly from said variety in the distinguishingcharacteristics above. Therefore, in one aspect, the disclosure providesa plant that does not differ significantly from lettuce NUN 06193 LTL inthe distinguishing characteristics above.

Similarity and differences between two different plant lines orvarieties can be determined by comparing the number of morphologicaland/or physiological characteristics that are the same (i.e.,statistically not significantly different) or that are different (i.e.,statistically significantly different) between the two plant lines orvarieties using plants grown under the same environmental conditions. Anumerical characteristic is considered to be “the same” when the valuefor a numeric characteristic is not significantly different at the 1%(p<0.01) or 5% (p<0.05) significance level, using one way analysis ofvariance (ANOVA), a standard method known to the skilled person.Non-numerical or “degree” or “type” characteristics are considered “thesame” when the values have the same “degree” or “type” when scored usingUSDA and/or UPOV descriptors, if the plants are grown under the sameenvironmental conditions.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Vegetative propagation”, “vegetative reproduction” or “clonalpropagation” are used interchangeably herein and mean a method of takinga plant part and inducing or allowing that plant part to form at leastroots, and also refer to the plant or plantlet obtained by that method.Optionally, the vegetative propagation is grown into a mature plant. Theskilled person is aware of what plant parts are suitable for use in themethod.

“Crossing” refers to the mating of two parent plants. The termencompasses “cross-pollination” and “selfing”.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms orsignificantly reduced symptoms to a specified biotic pest, pathogen,abiotic influence or environmental condition compared to a susceptibleplant. These terms are optionally also used to describe plants showingsome symptoms but that are still able to produce marketable product withan acceptable yield.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, doubled haploid production, embryo rescue,protoplast fusion, marker assisted selection, mutation breeding etc. asknown to the breeder (e.g., methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one lettuce lineor variety to another.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce a progeny plant.Progeny plants which have the trait are then crossed to the recurrentparent. After several generations of backcrossing and/or selfing therecurrent parent comprises the trait of the donor. The plant generatedin this way may be referred to as a “single trait converted plant”. Thetechnique can also be used on a parental line of a hybrid.

“Progeny” as used herein refers to a plant obtained from a plantdesignated NUN 06193 LTL. A progeny may be obtained by regeneration ofcell culture or tissue culture or parts of a plant of said variety orselfing of a plant of said variety or by producing seeds of a plant ofsaid variety. In further aspects, progeny may also encompass plantsobtained from crossing of at least one plant of said variety withanother lettuce plant of the same variety or another variety or line, orwith wild lettuce plants. A progeny may comprise a mutation or atransgene. A “first generation progeny” is the progeny directly derivedfrom, obtained from, obtainable from or derivable from the parent plantby, e.g., traditional breeding methods (selfing and/orcross-pollinating) or regeneration (optionally combined withtransformation or mutation). Thus, a plant of lettuce variety NUN 06193LTL is the male parent, the female parent or both of a first-generationprogeny of lettuce variety NUN 06193 LTL. Progeny may have all thephysiological and morphological characteristics of lettuce variety NUN06193 LTL when grown under the same environmental conditions. Usingmethods such as backcrossing, recurrent selection, mutation ortransformation, one or more specific characteristics may be introducedinto said variety, to provide or a plant comprising all but 1, 2, or 3of the morphological and physiological characteristics of lettucevariety NUN 06193 LTL.

The terms “gene converted” or “conversion plant” or “single locusconverted plant” in this context refer to lettuce plants which aredeveloped by traditional breeding techniques, e.g., backcrossing, or viagenetic engineering or through mutation breeding, wherein essentiallyall of the desired morphological and physiological characteristics ofthe parent variety or line are recovered, in addition to the one or morecharacteristics introduced into the parent via e.g., the backcrossingtechnique (optionally including reverse breeding or reverse synthesis ofbreeding lines). It is understood that not only the addition of afurther characteristic (e.g., addition of gene conferring a furthercharacteristic, such as a disease resistance gene), but also thereplacement/modification of an existing characteristic by a differentcharacteristic is encompassed herein (e.g., a mutant allele of a genecan modify the phenotype of a characteristic).

Likewise, a “Single Locus Converted (Conversion) Plant” refers to plantsdeveloped by plant breeding techniques comprising or consisting ofmutation breeding and/or by genetic transformation and/or by traditionalbreeding techniques, such as backcrossing, wherein essentially all ofthe desired morphological and physiological characteristics of a lettucevariety are recovered in addition to the characteristics of the singlelocus having been transferred into the variety via the above mentionedtechnique, or wherein a morphological and physiological characteristicof the variety has been replaced/modified in the variety. In case of ahybrid, the gene may be introduced, or modified, in the male or femaleparental line.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know suitable growing conditions for lettuce variety NUN06193 LTL. The mean, if not indicated otherwise within this application,refers to the arithmetic mean of measurements on at least 10 different,randomly selected plants of a variety or line.

DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE

The disclosure relates to a plant of lettuce variety NUN 06193 LTL,wherein a representative sample of seeds of said variety will bedeposited under the Budapest Treaty, with Accession number NCIMB 43665.NUN 06193 LTL is a green long-day Romaine lettuce of the open type, withsolid ribs.

The disclosure also relates to a seed of lettuce variety NUN 06193 LTL,wherein a representative sample of said seed will be deposited under theBudapest Treaty, with Accession number NCIMB 43665.

In another aspect, the disclosure provides for a plant part of varietylettuce variety NUN 06193 LTL, preferably a head or a leaf, arepresentative sample of seed from said variety will be deposited underthe Budapest Treaty, with Accession number NCIMB 43665.

A seed of inbred variety NUN 06193 LTL is obtainable by selfing thevariety and harvesting the seeds produced. The resultant seeds can begrown to produce plants of said variety. In one aspect, a seed or aplurality of seeds of said variety are packaged into a container of anysize or type (e.g., bags, cartons, cans, etc.). The seed may bedisinfected, primed and/or treated with various compounds, such as seedcoatings or crop protection compounds. The seed produces a plant oflettuce variety NUN 06193 LTL.

Also provided is a plant of lettuce variety NUN 06193 LTL, or a head ora leaf or other plant part thereof, produced from a seed, wherein arepresentative sample of said seeds will be deposited under the BudapestTreaty, with Accession Number NCIMB 43665.

Also provided is a plant part obtained from variety NUN 06193 LTL,wherein said plant part is a leaf, a part of a leaf, a head, a part of ahead, a fruit, a part of a fruit, pollen, an ovule, a cell, a petiole, ashoot or a part thereof, a stem or a part thereof, a root or a partthereof, a root tip, a cutting, a seed, a part of a seed, seed coat, oranother maternal tissue which is part of a seed grown on said variety,hypocotyl, cotyledon a pistil, an anther, or a flower or a part thereof.Heads and leaves are particularly important plant parts. In a furtheraspect, the plant part obtained from variety NUN 06193 LTL is a cell,optionally a cell in a cell or tissue culture. The cell may be growninto a plant of lettuce variety NUN 06193 LTL. A part of lettuce varietyNUN 06193 LTL (or of a progeny of that variety or of a plant having allphysiological and/or morphological characteristics but one, two or threeof lettuce variety NUN 06193 LTL) further encompasses any cells,tissues, organs obtainable from the seedlings or plants in any stage ofmaturity.

The disclosure also provides for a food or feed product or a processedproduct comprising or consisting of a plant part described herein.Preferably, the plant part is a lettuce head or leaf or part thereofand/or an extract from a leaf or another plant part described hereincomprising at least one cell of lettuce variety NUN 06193 LTL. The foodor feed product may be fresh or processed, e.g., dried, grinded,powdered, pickled, chopped, cooked, roasted, in a sauce, in a sandwich,pasted, puréed or concentrated, juiced, pickled, canned, steamed,boiled, fried, blanched and/or frozen, etc.

Such a plant part of lettuce variety NUN 06193 LTL can be stored and/orprocessed further. The disclosure thus also provides for a food or feedproducts comprising one or more of such parts, such as canned, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, frozen, dried, pickled, or powdered lettuce headsor leaves from variety NUN 06193 LTL or from progeny of said variety, orfrom a derived variety, such as a plant having all but one, two or threephysiological and/or morphological characteristics of lettuce varietyNUN 06193 LTL.

In another aspect, the disclosure provides for a lettuce head or leaf ofvariety NUN 06193 LTL, or a part of a head or leaf of said variety. Thehead or leaf can be in any stage of maturity, for example, immature ormature. In another aspect, the disclosure provides for a containercomprising or consisting of a plurality of harvested lettuce heads orleaves or parts of lettuce heads or leaves of said variety, or lettuceheads or leaves of progeny thereof, or lettuce heads or leaves of aderived variety.

Marketable lettuce heads or leaves are generally sorted by size andquality after harvest. Alternatively, the lettuce heads or leaves can besorted by leaf size, shape, texture, glossiness or color.

In another aspect, the plant, plant part or seed of lettuce variety NUN06193 LTL is inside one or more containers. For example, the disclosureprovides containers such as cans, boxes, crates, bags, cartons, ModifiedAtmosphere Packaging, films (e.g., biodegradable films), etc. comprisinga plant or a part of a plant (fresh and/or processed) or a seed oflettuce variety NUN 06193 LTL. In a particular aspect, the containercomprises a plurality of seeds of lettuce variety NUN 06193 LTL, or aplurality of plant parts of lettuce variety NUN 06193 LTL.

The disclosure further relates to a lettuce variety, referred to as NUN06193 LTL, which when compared to Reference Variety NUN 06109 LTL hasthe following distinguishing characteristics as shown in Table 4: 1)taller plant height; 2) narrower spread of frame leaves; 3) narrowerwidth of mature leaf; 4) longer length/width index of mature leaf; 5) anentire indentation of mature leaf; 6) absence of undulations of theapical margin in the mature leaf; 7) smaller size of mature leaf; 8)duller mature leaf; 9) absence of blistering in the mature leaf; 10)thinner mature leaf; and 11) smaller size of plant head, where thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. Also encompassed areparts of that plant.

In one aspect, a plant of lettuce variety NUN 06193 LTL or a progenyplant thereof, comprises all of the following morphological and/orphysiological characteristics (i.e., average values of distinguishingcharacteristics, as indicated on the USDA Objective description ofvariety—lettuce (unless indicated otherwise)) as shown in Tables 1, 2,and 3: 1) taller plant height; 2) narrower spread of frame leaves; 3)narrower width of mature leaf; 4) longer length/width index of matureleaf; 5) an entire indentation of mature leaf; 6) absence of undulationsof the apical margin in the mature leaf; 7) smaller size of mature leaf;8) duller mature leaf; 9) absence of blistering in the mature leaf; 10)thinner mature leaf; and 11) smaller size of plant head, where thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. An example of values forthe distinguishing characteristics collected in a trial can be found,for example, in Table 4. A part of this plant is also provided.

In another aspect, a plant of lettuce variety NUN 06193 LTL or a partthereof comprises resistance to Downy Mildew (Bremia lactucae) isolatesB1:16-28 and 31-32 EU, Lettuce Mosaic Virus (LMV) Strain Ls 1, andRhizomonas subefaciens (Corky Root), measured according to UPOVstandards described in TG/13/11.

The disclosure further provides a lettuce plant which does not differfrom the physiological and morphological characteristics of the plant oflettuce variety NUN 06193 LTL, as determined at the 1%, 2%, 3%, 4% or 5%significance level, when grown under the same environmental conditions.In a particular aspect, the plants are measured in the same trial (e.g.,the trial is conducted as recommended by the USDA or UPOV). Thedisclosure also comprises a part of said plant, preferably a fruit or apart thereof.

The disclosure also provides a tissue or cell culture comprising cellsof lettuce variety NUN 06193 LTL. Such a tissue culture can, forexample, be grown on plates or in liquid culture or be frozen for longterm storage. The cells of lettuce variety NUN 06193 LTL used to startthe culture can be any plant part suitable for vegetative reproduction,or, in a particular aspect, can be one or more of an embryo, meristem, acotyledon, a hypocotyl, pollen, a leaf, an anther, a root, a root tip, apistil, a petiole, a flower, a fruit, seed, or a stem. In anotherparticular aspect, the tissue culture does not contain somaclonalvariation or has reduced somaclonal variation. The skilled person isfamiliar with methods to reduce or prevent somaclonal variation,including regular reinitiation.

In one aspect, the disclosure provides a lettuce plant regenerated fromthe tissue or cell culture of variety NUN 06193 LTL, wherein theregenerated plant is not significantly different from lettuce varietyNUN 06193 LTL in all, or all but one, two or three, of the physiologicaland morphological characteristics (determined at the 5% significancelevel when grown under the same environmental conditions). Optionally,the plant has one, two or three the physiological and morphologicalcharacteristics that are affected by a mutation or by transformation. Inanother aspect, the disclosure provides a lettuce plant regenerated fromthe tissue or cell culture of variety NUN 06193 LTL, wherein the planthas all of the physiological and morphological characteristics of saidvariety determined at the 5% significance level when grown under thesame environmental conditions. Similarity or difference of acharacteristic is determined by measuring the characteristics on arepresentative number of plants grown under the same environmentalconditions, determining whether type/degree characteristics are the sameand determining whether numerical characteristics are different at the5% significance level.

Lettuce variety NUN 06193 LTL, or its progeny, or a plant having allphysiological and/or morphological characteristics but one, two or threewhich are different from those of lettuce variety NUN 06193 LTL, canalso be reproduced using vegetative reproduction methods. Therefore, thedisclosure provides for a method of producing a plant or plant part oflettuce variety NUN 06193 LTL, comprising vegetative propagation oflettuce variety NUN 06193 LTL. Vegetative propagation comprisesregenerating a whole plant from a plant part of lettuce variety NUN06193 LTL or from a progeny or from or a plant having all physiologicaland/or morphological characteristics of said variety but one, two orthree different characteristics, such as a cutting, a cell culture or atissue culture.

The disclosure also provides methods of vegetatively propagating a partof the plant of the variety NUN 06193 LTL. In certain aspects, themethod comprises: (a) cultivating tissue or cells capable of beingpropagated from lettuce variety NUN 06193 LTL to obtain proliferatedshoots; and (b) rooting said proliferated shoots, to obtain rootedplantlets. Steps (a) and (b) may also be reversed, i.e., firstcultivating said tissue to obtain roots and then cultivating the tissueto obtain shoots, thereby obtaining rooted plantlets. The rootedplantlets may then be further grown, to obtain plants. In one aspect,the method further comprises step (c) growing plants from said rootedplantlets. Therefore, the method also comprises regenerating a wholeplant from a part of lettuce variety NUN 06193 LTL. In a particularaspect, the part of the plant to be propagated is a cutting, a cellculture or a tissue culture.

The disclosure also provides for a vegetatively propagated plant ofvariety NUN 06193 LTL (or from progeny of NUN 06193 LTL or from or aplant having all but one, two or three physiological and/ormorphological characteristics of lettuce variety NUN 06193 LTL), whereinthe plant has all of the morphological and physiological characteristicsof NUN 06193 LTL when the characteristics are determined at the 5%significance level for plants grown under the same conditions. Inanother aspect, the propagated plant has all but one, two or three ofthe morphological and physiological characteristics of lettuce varietyNUN 06193 LTL when the characteristics are determined at the 5%significance level for plants grown under the same conditions. A part ofsaid propagated plant or said propagated plant with one, two or threedifferences is also provided.

In another aspect, the disclosure provides a method for producing aplant part, preferably a head or leaf, comprising growing a plant oflettuce variety NUN 06193 LTL until it develops at least one leaf ordevelops a head, and optionally collecting the head or leaf. Preferably,the head or leaf is collected at harvest maturity. In another aspect,the leaf is collected at baby leaf stage. A plant of lettuce variety NUN06193 LTL can be produced by seeding directly in the soil (e.g., field)or by germinating the seeds in controlled environment conditions (e.g.,greenhouses, hydroponic cultures, etc.) and optionally thentransplanting the seedlings into the field (see, e.g., Gonai et al., J.Exp. Bot., 55(394): 111, 2004; http://anrcatalog.ucanr.edu/pdf/7215.pdf;http://anrcatalog.ucanr.edu/pdf/7216.pdf). Lettuce may also be grown intunnels. Moreover, said variety can be grown in hydroponic cultures asdescribed in, e.g., US2008/0222949, which is herein incorporated byreference in its entirety, and the skilled person is familiar withvarious types of hydroponic cultures. Alternatively, seed of lettucevariety NUN 06193 LTL may be grown on peat block for use as root balllettuce. Furthermore, lettuce variety NUN 06193 LTL may be combined with1, 2 or 3 other lettuce varieties to be grown as “composite lettuce”(see, e.g., EP1197137, which is herein incorporated by reference in itsentirety).

In still another aspect, the disclosure provides a method of producing alettuce plant, comprising crossing a plant of variety NUN 06193 LTL witha second lettuce plant at least once, allowing seed to develop andoptionally harvesting said progeny seed. The skilled person can selectprogeny from said crossing. Optionally, the progeny (grown from theprogeny seed) is crossed twice, thrice, or four, five, six or seventimes, and allowed to set seed. In one aspect, the first “crossing”further comprises planting seeds of a first and a second parent lettuceplant, often in proximity so that pollination will occur; for example,mediated by insect vectors. Alternatively, pollen can be transferredmanually. Where the plant is self-pollinated, pollination may occurwithout the need for direct human intervention other than plantcultivation. After pollination, the plant can produce seed.

In yet another aspect, the disclosure provides a method of producing aplant, comprising selfing a plant of variety NUN 06193 LTL one or moretimes, and selecting a progeny plant from said selfing. In one aspect,the progeny plant retains all or all but one, two or three of thephysiological and morphological characteristics of lettuce variety NUN06193 LTL described above when grown under the same environmentalconditions. In a different aspect, the progeny plant comprises all ofthe physiological and morphological characteristic of lettuce varietyNUN 06193 LTL of Tables 1, 2, and 3.

In other aspects, the disclosure provides a progeny plant of variety NUN06193 LTL such as a progeny plant obtained by further breeding oflettuce variety NUN 06193 LTL. Further breeding with lettuce variety NUN06193 LTL includes selfing that variety and/or cross-pollinating varietyNUN 06193 LTL with another lettuce plant one or more times. Inparticular, the disclosure provides for a progeny plant that retains allthe morphological and physiological characteristics of lettuce varietyNUN 06193 LTL or, in another aspect, a progeny plant that retains all,or all but one, two or three, of the morphological and physiologicalcharacteristics of lettuce variety NUN 06193 LTL, optionally all or allbut one, two or three of the characteristics as listed in Tables 1, 2,and 3, determined at the 5% significance level for numericalcharacteristics, when grown under the same environmental conditions. Inanother aspect, the progeny is a first-generation progeny, e.g., theovule or the pollen (or both) used in the crossing is an ovule or pollenof lettuce variety NUN 06193 LTL, where e.g. the pollen comes from ananther of lettuce variety NUN 06193 LTL and/or the ovule comes from anovary of lettuce variety NUN 06193 LTL. In another aspect, thedisclosure provides for a vegetative reproduction of the variety and aplant having all, or all but 1, 2, or 3 of the physiological andmorphological characteristics of lettuce variety NUN 06193 LTL (e.g., aslisted in Tables 1, 2, and 3).

The disclosure also provides a method for collecting pollen of lettucevariety NUN 06193 LTL, comprising collecting pollen from a plant ofvariety NUN 06193 LTL. Alternatively, the method comprises growing aplant of lettuce variety NUN 06193 LTL until at least one flowercontains pollen and collecting the pollen. In a particular aspect, thepollen is collected when it is mature or ripe. A suitable method forcollecting pollen comprises collecting anthers or the part of the antherthat contains pollen, for example, by cutting the anther or the part ofthe anther off. Pollen can be collected in a container. Optionally,collected pollen can be used to pollinate a lettuce flower.

The morphological and/or physiological differences between two differentindividual plants described herein (e.g., between lettuce variety NUN06193 LTL and a progeny of lettuce variety NUN 06193 LTL) or between aplant of lettuce variety NUN 06193 LTL or progeny of lettuce variety NUN06193 LTL, or a plant having all, or all but 1, 2, or 3, of thephysiological and morphological characteristics of lettuce variety NUN06193 LTL (or all, or all but 1, 2, or 3 of the characteristics aslisted in Tables 1, 2, and 3) and another known variety can easily beestablished by growing said variety next to each other or next to theother variety (e.g., in the same field, under the same environmentalconditions), preferably in several locations which are suitable for saidlettuce cultivation, and measuring morphological and/or physiologicalcharacteristics of a number of plants (e.g., to calculate an averagevalue and to determine the variation range/uniformity within thevariety). For example, trials can be carried out in Acampo Calif., USA(N 38 degrees 07′261″/W 121 degrees 18′807″, USA), whereby variouscharacteristics, for example, maturity, leaf shape, size and texture,leaf color and glossiness, bolt shape, surface and length, flower sizeand color, head weight, disease resistance, insect resistance andresistance to physiological stress, can be measured and directlycompared for species of lettuce. Thus, the disclosure comprises lettuceplant having one, two or three physiological and/or morphologicalcharacteristics which are different from those of the plant of lettucevariety NUN 06193 LTL, and which otherwise has all the physiological andmorphological characteristics of the plant of lettuce variety NUN 06193LTL, when determined at the 5% significance level for plants grown underthe same environmental conditions. In one aspect, the differentcharacteristic(s) is/are a result of breeding with lettuce variety NUN06193 LTL and selection of a progeny plant comprising 1, 2 or 3characteristics which are different than in lettuce variety NUN 06193LTL. In another aspect, the different characteristic is the result of amutation (e.g., a spontaneous mutation or a human induced mutationthrough e.g., targeted mutagenesis or traditional mutagenesis such aschemically or radiation induced mutagenesis), or it is the result oftransformation.

The morphological and physiological characteristics (and thedistinguishing characteristics) of lettuce variety NUN 06193 LTL areprovided, for example, in Tables 1, 2, and 3. Encompassed herein is alsoa plant obtainable from lettuce variety NUN 06193 LTL (e.g., by selfingand/or crossing and/or backcrossing with said variety and/or progeny ofsaid variety) comprising all or all but one, two or three of thephysiological and morphological characteristics of lettuce variety NUN06193 LTL listed in Tables 1, 2, and 3 as determined at the 5%significance level for numerical characteristics or identical fornon-numerical characteristics when grown under the same environmentalconditions and/or comprising one or more (or all; or all except one, twoor three) characteristics when grown under the same environmentalconditions. The morphological and/or physiological characteristics mayvary somewhat with variation in the environment (such as temperature,light intensity, day length, humidity, soil, fertilizer use), which iswhy a comparison under the same environmental conditions is preferred.Colors can best be measured using the Royal Horticultural Society (RHS)Chart.

In yet another aspect, the disclosure provides for a method of producinga new lettuce plant. The method comprises crossing lettuce variety NUN06193 LTL, or a plant comprising all but 1, 2, or 3 of the morphologicaland physiological characteristics of lettuce variety NUN 06193 LTL (aslisted in Tables 1, 2, and 3), or a progeny thereof, either as male oras female parent, with a second lettuce plant (or a wild relative oflettuce) one or more times, and/or selfing a lettuce plant of lettucevariety NUN 06193 LTL, or a progeny plant thereof, one or more times,and selecting progeny from said crossing and/or selfing. The secondlettuce plant may, for example, be a line or variety of the speciesLactuca sativa or even another Lactuca species.

The disclosure provides for methods of producing plants which retain allthe morphological and physiological characteristics of a plant describedherein. The disclosure also provides for methods of producing a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of lettuce variety NUN 06193 LTL (e.g., aslisted in Tables 1, 2, and 3), but which are still genetically closelyrelated to said variety. The relatedness can, for example, be determinedby fingerprinting techniques (e.g., making use of isozyme markers and/ormolecular markers such as Single-nucleotide polymorphism (SNP) markers,amplified fragment length polymorphism (AFLP) markers, microsatellites,minisatellites, Random Amplified Polymorphic DNA (RAPD) markers,restriction fragment length polymorphism (RFLP) markers and others). Aplant is “closely related” to lettuce variety NUN 06193 LTL if its DNAfingerprint is at least 80%, 90%, 95% or 98% identical to thefingerprint of NUN 06193 LTL. In a particular aspect, AFLP markers areused for DNA fingerprinting (see, e.g., Vos et al. 1995, Nucleic AcidResearch 23: 4407-4414). A closely related plant may have a Jaccard'sSimilarity index of at least about 0.95 or 0.96 or more (see, e.g.,“Guidelines for the Handling of a Dispute on Essential Derivation inLettuce” at worldseed.org/wp-content/uploads/2015/10/GuidelinesEDV_Lettuce_2004.pdf). The disclosure also provides a plant and avariety obtained or selected by applying these methods on lettucevariety NUN 06193 LTL. Such a plant may be produced by crossing and/orselfing, or alternatively, a plant may simply be identified and selectedamongst plants of said variety, or progeny of said variety, e.g., byidentifying a variant within lettuce variety NUN 06193 LTL or withinprogeny of said variety (e.g., produced by selfing) which variantdiffers from lettuce variety NUN 06193 LTL in one, two or three of themorphological and/or physiological characteristics (e.g.,characteristics listed in Tables 1, 2, and 3). In one aspect, thedisclosure provides a plant of lettuce variety NUN 06193 LTL having aJaccard's Similarity index with said variety of at least 0.95, 0.96,0.97, 0.98 or even at least 0.99.

In some aspects, the disclosure provides a lettuce plant comprisinggenomic DNA having at least 95%, 96%, 97%, 98% or 99% sequence identitycompared to the genomic DNA sequence of a plant of lettuce variety NUN06193 LTL as deposited under Accession Number NCIMB 43665. In someaspects, the lettuce plant further comprises all or all but 1, 2, or 3of the morphological and physiological characteristics of lettucevariety NUN 06193 LTL (e.g., as listed in Tables 1, 2, and 3). In otheraspects, the lettuce plant comprises the distinguishing characteristicsof lettuce variety NUN 06193 LTL.

For the purpose of this disclosure, the “sequence identity” ofnucleotide sequences, expressed as a percentage, refers to the number ofpositions in the two optimally aligned sequences which have identicalresidues (×100) divided by the number of positions compared. A gap,i.e., a position in the pairwise alignment where a residue is present inone sequence but not in the other, is regarded as a position withnon-identical residues. A pairwise global sequence alignment of twonucleotide sequences is found by aligning the two sequences over theentire length according to the Needleman and Wunsch global alignmentalgorithm described in Needleman and Wunsch, 1970, J. Mol. Biol.48(3):443-53. A full implementation of the Needleman-Wunsch globalalignment algorithm is found in the needle program in The EuropeanMolecular Biology Open Software Suite (see, EMBOSS, Rice et al., Trendsin Genetics June 2000, vol. 16, no. 6. pp. 276-277).

The disclosure also provides methods for determining the identity ofparental lines of plants described herein, in particular the identity ofthe female line. US 2015/0126380, which is hereby incorporated byreference, relates to a non-destructive method for analyzing maternalDNA of a seed. In this method, the DNA is dislodged from the seed coatsurface and can be used to collect information on the genome of thematernal parent of the seed. This method for analyzing maternal DNA of aseed comprises contacting a seed with a fluid to dislodge DNA from theseed coat surface, and analyzing the DNA thus dislodged from the seedcoat surface using methods known in the art. The skilled person is thusable to determine whether a seed has grown on a plant of a plant oflettuce variety NUN 06193 LTL or is a progeny of said variety, becausethe seed coat of the seed is a maternal tissue genetically identical tolettuce variety NUN 06193 LTL. Since lettuce variety NUN 06193 LTL is aninbred variety, with a very high degree of homozygosity, any F1 progenywill inherit the same, predictable, set of chromosomes from its parent.Thus, the skilled person will also be able to identify maternal tissuesof a seed grown on a F1 progeny of lettuce variety NUN 06193 LTL, usingthe methods described in US 2015/0126380. In another particular aspect,the skilled person can determine the identity of the female parentalline of lettuce variety NUN 06193 LTL by analyzing the seed coat of aseed of that variety. In another aspect, the skilled person candetermine whether a seed is grown on lettuce variety NUN 06193 LTL.

By crossing and/or selfing (one or more) single traits may be introducedinto, or modified in, lettuce variety NUN 06193 LTL (e.g., usingbackcrossing breeding schemes), while retaining the remainingmorphological and physiological characteristics of said variety and/orwhile retaining one or more or all distinguishing characteristics. Asingle trait converted plant may thereby be produced. For example,disease resistance genes may be introduced, genes responsible for one ormore quality traits, yield, etc. Both single genes (e.g., dominant orrecessive) and one or more QTLs (quantitative trait loci) may betransferred into lettuce variety NUN 06193 LTL by breeding with saidvariety.

Any pest or disease resistance genes may be introduced into lettucevariety NUN 06193 LTL, progeny of lettuce variety NUN 06193 LTL or intoa plant comprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of lettuce variety NUN 06193 LTL (e.g., aslisted in Tables 1, 2, and 3). Resistance to one or more of thefollowing diseases or pests may be introduced into plants describedherein: Rhizomonas suberifaciens (Corky root rot), Bremia lactutae(Downy mildew), Erysiphe cichoracearum f. sp. lactutae (Powdery mildew),Sclerotinia minor and Sclerotinia sclerotiorum (Lettuce Drop),Pseudomonas spp. (Bacterial Soft Rot), Botrytis cinerea (Grey Mold),Verticillium dahlia (Verticillium Wilt), Xanthomonas spp. (BacterialLeaf Spot), Microdochium panattonianum (Anthracnose), Fusarium oxysporumf. sp. lactutae, Rhizoctonia solani (Bottom Rot), Cabbage Loopers,Lettuce Root Aphid, Myzus persicae (Green Peach Aphid), Liriomyza langei(Pea Leafminer), Liriomyza trifolii (Serpentine Leafminer), Liriomyzasativae (Vegetable Leafminer), Foxglove Aphid, Potato Aphid, BeetArmyworm, Bemisia argentifolii (Silver Whitefly), and/or Aster Yellows.Other resistance genes, against pathogenic viruses (e.g., MirafioriLettuce Big Vein Virus (LMBVV), Lettuce Infectious Yellows Virus (LIYV),Lettuce Mosaic Virus (LMV), Lettuce Necrotic Stunt Virus (LNSV),Cucumber Mosaic Virus (CMV), Tomato Bushy Stunt Virus (Dieback), TomatoSpotted Wilt Virus (TSWV), Turnip Mosaic Virus, Beet Western YellowsVirus (BWYV), Alfalfa mosaic virus (AMV)), fungi, bacteria, nematodes,insects or other pests may also be introduced. In one aspect, resistanceagainst Nasonovia ribisnigri biotype Nr:0 and/or Nr:1 may be introducedinto the plant disclosed herein. Also, any resistances to physiologicalstresses may be introduced into a plant described herein, or progenythereof or into a plant comprising all but 1, 2, or 3 or more of themorphological and physiological characteristics of said plant (e.g., aslisted in Tables 1, 2, and 3). Resistance against one or more of thefollowing may be introduced into plants described herein: Tip burn,Heat, Drought, Cold, Salt and/or Brown rob (Rib Discoloration/RibBlight).

The disclosure also provides a method for developing a lettuce plant ina lettuce breeding program, using a lettuce plant described herein, orits parts as a source of plant breeding material. Suitable plantbreeding techniques are recurrent selection, backcrossing, pedigreebreeding, mass selection, mutation breeding and/or genetic markerenhanced selection. In one aspect, the method comprises crossing lettucevariety NUN 06193 LTL or progeny of said variety, or a plant comprisingall but 1, 2, or 3 or more of the morphological and physiologicalcharacteristics of lettuce variety NUN 06193 LTL (e.g., as listed inTables 1, 2, and 3), with a different lettuce plant, and wherein one ormore offspring of the crossing are subject to one or more plant breedingtechniques: recurrent selection, backcrossing, pedigree breeding, massselection, mutation breeding and genetic marker enhanced selection (seee.g., Martin et al. 2008, Australian Journal of Crop Science 1(2):43-46). For breeding methods in general, see, e.g., Principles of PlantGenetics and Breeding, 2007, George Acquaah, Blackwell Publishing,ISBN-13: 978-1-4051-3646-4.

The disclosure also provides a lettuce plant comprising at least a firstset of the chromosomes of lettuce variety NUN 06193 LTL, a sample ofseed of said variety will be deposited under Accession Number NCIMB43665; optionally further comprising a single locus conversion or amutation, wherein said plant has essentially all of the morphologicaland physiological characteristics of the plant comprising at least afirst set of the chromosomes of said variety. In another aspect, thissingle locus conversion or mutation confers a trait, wherein the traitis yield, nutritional value, taste, color, crunchiness, male sterility,herbicide tolerance, insect resistance, pest resistance, diseaseresistance, environmental stress tolerance, modified carbohydratemetabolism and/or modified protein metabolism.

In one aspect, a plant of lettuce variety NUN 06193 LTL may also bemutated (by e.g., irradiation, chemical mutagenesis, heat treatment,etc.) and mutated seeds or plants may be selected in order to change oneor more characteristics of said variety. Methods such as TILLING may beapplied to lettuce populations in order to identify mutants. Similarly,lettuce variety NUN 06193 LTL may be transformed and regenerated,whereby one or more chimeric genes are introduced into the variety orinto a plant comprising all but 1, 2, 3, or more of the morphologicaland physiological characteristics (e.g., as listed in Tables 1, 2, and3). Transformation can be carried out using standard methods, such asAgrobacterium tumefaciens mediated transformation or biolistics,followed by selection of the transformed cells and regeneration intoplants. A desired trait (e.g., gene(s) conferring pest or diseaseresistance, herbicide, fungicide or insecticide tolerance, etc.) can beintroduced into lettuce variety NUN 06193 LTL, or progeny of saidvariety, by transforming said variety or progeny of said variety with atransgene that confers the desired trait, wherein the transformed plantretains all or all but one, two or three of the phenotypic and/ormorphological and/or physiological characteristics of lettuce varietyNUN 06193 LT L or the progeny of said variety and contains the desiredtrait. In another aspect, the transformation or mutation confers atrait, wherein the trait is yield, nutritional value, taste, color,crunchiness, male sterility, herbicide tolerance, insect resistance,pest resistance, disease resistance, environmental stress tolerance,modified carbohydrate metabolism and/or modified protein metabolism.

The disclosure also provides a plant or a cell of a plant comprising adesired trait produced by mutating a seed or plant of variety NUN 06193LTL or a cell thereof and selecting a plant the desired trait, whereinthe mutated plant retains all or all but one of the phenotypic andmorphological characteristics of said variety, optionally as describedfor each variety in Tables 1, 2, and 3, and contains the desired traitand wherein a representative sample of seed of variety NUN 06193 LTLwill be deposited under Accession Number NCIMB 43665. In a furtheraspect, the desired trait is yield, nutritional value, taste, color,crunchiness, male sterility, herbicide tolerance, insect resistance,pest resistance, disease resistance, environmental stress tolerance,modified carbohydrate metabolism and/or modified protein metabolism.

In one aspect, the disclosure provides a method for inducing mutation inlettuce variety NUN 06193 LTL comprising:

-   -   a. exposing the seed, the plant or the plant part or the cell of        lettuce variety NUN 06193 LTL to a mutagenic compound or to        radiation, wherein a representative sample of seed of variety        NUN 06193 LTL will be deposited under Accession Number NCIMB        43665;    -   b. selecting the seed, the plant or the plant part or the cell        of lettuce variety NUN 06193 LTL having a mutation; and    -   c. optionally growing and/or multiplying the seed, plant or        plant part or cell of lettuce variety NUN 06193 LTL having the        mutation.

The disclosure also provides a plant having one, two or threephysiological and/or morphological characteristics which are differentfrom those of lettuce variety NUN 06193 LTL, and which otherwise has allthe physiological and morphological characteristics of said variety,wherein a representative sample of seed of variety NUN 06193 LTL will bedeposited under Accession Number NCIMB 43665. In particular, variantswhich differ from lettuce variety NUN 06193 LTL in none, one, two orthree of the characteristics mentioned in Tables 1, 2, and 3 areencompassed.

A part of lettuce variety NUN 06193 LTL (or of progeny of said varietyor of a plant having all physiological and/or morphologicalcharacteristics but one, two or three which are different from those ofsaid variety) encompasses any cells, tissues, organs obtainable from theseedlings or plants, such as but not limited to: a lettuce leaf or apart thereof, a lettuce head, a cutting, hypocotyl, cotyledon, seedcoat, pollen and the like. Such parts can be stored and/or processedfurther. The disclosure further provides for food or feed productscomprising a part of lettuce variety NUN 06193 LTL or a part of progenyof lettuce variety NUN 06193 LTL, or a part of a plant having all butone, two or three physiological and/or morphological characteristics oflettuce variety NUN 06193 LTL, comprising one or more of such parts,optionally processed (such as canned, chopped, cooked, roasted, in asauce, in a sandwich, pasted, puréed or concentrated, juiced, frozen,dried, pickled, or powdered).

In one aspect, the disclosure provides for a haploid plant and/or adoubled haploid plant of lettuce variety NUN 06193 LTL, or of a planthaving all but one, two or three physiological and/or morphologicalcharacteristics of lettuce variety NUN 06193 LTL, or progeny of any ofthese, is encompassed herein. Haploid and doubled haploid (DH) plantscan, for example, be produced by cell or tissue culture and chromosomedoubling agents and regeneration into a whole plant. DH productionchromosome doubling may be induced using known methods, such ascolchicine treatment or the like. In one aspect, the method comprisesinducing a cell or tissue culture with a chromosome doubling agent andregenerating the cells or tissues into a whole plant.

In another aspect, the disclosure comprises a method for making doubledhaploid cells from haploid cells of lettuce variety NUN 06193 LTLcomprising doubling cells of lettuce variety NUN 06193 LTL with achromosome doubling agent, such as colchicine treatment (see, e.g.,Nikolova and Niemirowicz-Szczytt (1996) Acta Soc Bot Pol 65:311-317).

In another aspect, the disclosure provides for haploid plants and/ordoubled haploid plants derived from NUN 06193 LTL that, when combined,make a set of parents of lettuce variety NUN 06193 LTL. The haploidplant and/or the doubled haploid plant of variety NUN 06193 LTL can beused in a method for generating parental lines of lettuce variety NUN06193 LTL.

Using methods known in the art such as “reverse synthesis of breedinglines” or “reverse breeding”, it is possible to produce parental linesfor a hybrid plant such as lettuce variety NUN 06193 LTL. A skilledperson can take any individual heterozygous plant (called a“phenotypically superior plant” in Example 2 of US2015/0245570 herebyincorporated by reference in its entirety; lettuce variety NUN 06193 LTLis such plant) and generate a combination of parental lines (reversebreeding parental lines) that, when crossed, produce the variety NUN06193 LTL. It is not necessary that the reverse breeding parental linesare identical to the original parental lines. Such new breeding methodsare based on the segregation of individual alleles in the sporesproduced by a desired plant and/or in the progeny derived from theself-pollination of that desired plant, and on the subsequentidentification of suitable progeny plants in one generation, or in alimited number of inbred cycles. Such a method is known fromUS2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9,Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049. Thus, thedisclosure provides a method for producing parental lines for a hybridorganism (e.g., lettuce variety NUN 06193 LTL), comprising in oneaspect: a) defining a set of genetic markers present in a heterozygousform (H) in a partially heterozygous starting organism; b) producingdoubled haploid lines from spores of the starting organism; c)genetically characterizing the doubled haploid lines thus obtained forthe said set of genetic markers to determine whether they are present ina first homozygous form (A) or in a second homozygous form (B); and d)selecting at least one pair of doubled haploid lines that havecomplementary alleles for at least a subset of the genetic markers,wherein each member of the pair is suitable as a parental line for thehybrid organism.

In another aspect, the method for producing parental lines for hybridorganisms, e.g., of lettuce variety NUN 06193 LTL, which when crossedreconstitute the genome of lettuce variety NUN 06193 LTL, comprising:

-   -   a) defining a set genetic markers that are present a        heterozygous form (H) in a partially heterozygous starting        organism;    -   b) producing at least one further generation from the starting        organism by self-pollination (e.g., F2 or F3 generation);    -   c) selecting at least one pair of progeny organisms in which at        least one genetic marker from the set is present in a        complementary homozygous forms (B vs. A, or A vs. B); and    -   d) optionally repeating steps b) and c) until at least one pair        of progeny organisms that have complementary alleles for at        least a subset of the genetic markers has been selected as        parental lines for a hybrid.

The disclosure relates to a method of producing a combination ofparental lines of a plant of lettuce variety NUN 06193 LTL, comprisingmaking doubled haploid cells from haploid cells from said plant or aseed of that plant; and optionally crossing these parental lines toproduce and collecting seeds. In another aspect, the disclosure relatesto a combination of parental lines produced by this method. In stillanother aspect the combination of parental lines can be used to producea seed or plant of lettuce variety NUN 06193 LTL, when these parentallines are crossed. In still another aspect, the disclosure relates to acombination of parental lines from which a seed or plant having allphysiological and/or morphological characteristics of lettuce varietyNUN 06193 LTL (when the characteristics are determined at the 5%significance level for plants grown under the same conditions).

The disclosure also provides a combination of parental lines which, whencrossed, produce a seed or plant having all physiological and/ormorphological characteristics of lettuce variety NUN 06193 LTL, but one,two or three which are different (when grown under the sameenvironmental conditions), as well as a seed or plant having allphysiological and/or morphological characteristics of lettuce varietyNUN 06193 LTL, but one, two or three which are different (when thecharacteristics are determined at the 5% significance level for plantsgrown under the same conditions).

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto lettuce variety NUN 06193 LTL, comprising:

-   -   a) obtaining a combination of a parental lines of lettuce        variety NUN 06193 LTL, optionally through reverse synthesis of        breeding lines;    -   b) introducing a single locus conversion in at least one of the        parents of step a; and    -   c) crossing the converted parent with the other parent of step a        to obtain seed lettuce variety NUN 06193 LTL.

In another aspect, a combination of a male and a female parental line oflettuce variety NUN 06193 LTL can be generated by methods describedherein, for example, through reverse synthesis of breeding lines.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto lettuce variety NUN 06193 LTL, comprising introducing a singlelocus conversion in at least one of the parents of lettuce variety NUN06193 LTL, and crossing the converted parent with the other parent oflettuce variety NUN 06193 LTL, to obtain seed of said variety.

In another aspect, introducing a single locus conversion in at least oneof the parent plants comprises:

-   -   a) obtaining a cell or tissue culture of cells of the parental        line of lettuce variety NUN 06193 LTL;    -   b) genetically transforming or mutating said cells;    -   c) growing the cells into a plant; and    -   d) optionally selecting plants that contain the single locus        conversion, the single trait conversion or the desired trait.

In another method, the step of introducing a single locus conversion inat least one of the parents comprises genetically transforming ormutating cells the parental line of lettuce variety NUN 06193 LTL,growing the cells into a plant; and optionally selecting plants thatcontain the single locus conversion, the single trait conversion or thedesired trait.

In another embodiment, the step of introducing a single locus conversionin at least one of the parent plants comprises:

-   -   a) crossing the parental line of lettuce variety NUN 06193 LTL,        with a second lettuce plant comprising the single locus        conversion, the single trait conversion or the desired trait;    -   b) selecting F1 progeny plants that contain the single locus        conversion, the single trait conversion or the desired trait;    -   c) crossing said selected progeny plants of step b) with the        parental line of step a) to produce a backcross progeny plant;    -   d) selecting backcross progeny plants comprising the single        locus conversion, the single trait conversion or the desired        trait and otherwise all or all but one, two or three of the        morphological and physiological characteristics the parental        line of step i to produce selected backcross progeny plants; and    -   e) optionally repeating steps c) and d) one or more times in        succession to produce selected second, third or fourth or higher        backcross progeny plants comprising the single locus conversion,        the single trait conversion or the desired trait and otherwise        all or all but one, two or three of the morphological and        physiological characteristics the parental line of step a) to        produce selected backcross progeny plants, when grown in the        same environmental conditions.        The disclosure further relates to plants obtained by this        method.

In any of the above methods, where the single locus conversion concernsa trait, the trait may be yield or pest resistance or diseaseresistance. In one aspect, the trait is disease resistance and theresistance is conferred to Rhizomonas suberifaciens (Corky root rot),Bremia lactutae (Downy mildew), Erysiphe cichoracearum f sp. lactutae(Powdery mildew), Sclerotinia minor and Sclerotinia sclerotiorum(Lettuce Drop), Pseudomonas spp. (Bacterial Soft Rot), Botrytis cinerea(Grey Mold), Verticillium dahlia (Verticillium Wilt), Xanthomonas spp.(Bacterial Leaf Spot), Microdochium panattonianum (Anthracnose),Fusarium oxysporum f. sp. lactutae, Rhizoctonia solani (Bottom Rot),Cabbage Loopers, Lettuce Root Aphid, Myzus persicae (Green Peach Aphid),Liriomyza langei (Pea Leafminer), Liriomyza trifolii (SerpentineLeafminer), Liriomyza sativae (Vegetable Leafminer), Foxglove Aphid,Potato Aphid, Beet Armyworm, Bemisia argentifolii (Silver Whitefly),and/or Aster Yellows. Other resistance genes, against pathogenic viruses(e.g., Mirafiori Lettuce Big Vein Virus (LMBVV), Lettuce InfectiousYellows Virus (LIYV), Lettuce Mosaic Virus (LMV), Lettuce Necrotic StuntVirus (LNSV), Cucumber Mosaic Virus (CMV), Tomato Bushy Stunt Virus(Dieback), Tomato Spotted Wilt Virus (TSWV), Turnip Mosaic Virus, BeetWestern Yellows Virus (BWYV), Alfalfa mosaic virus (AMV)), fungi,bacteria, nematodes, insects or other pests may also be introduced. Inone aspect, resistance against Nasonovia ribisnigri biotype Nr:0 and/orNr:1 maybe introduced into the plant disclosed herein. Also, anyresistances to physiological stresses may be introduced into a plantdescribed herein, or progeny thereof or into a plant comprising all but1, 2, or 3 or more of the morphological and physiologicalcharacteristics of said plant (e.g., as listed in Tables 1, 2, and 3).Resistance against one or more of the following may also be introducedinto plants of the disclosure: Tip burn, Heat, Drought, Cold, Saltand/or Brown Rob (Rib Discoloration/Rib Blight).

Also provided is a plant part obtainable from variety NUN 06193 LTL orfrom progeny of said variety or from a plant having all but one, two orthree physiological and/or morphological characteristics which aredifferent from those of lettuce variety NUN 06193 LTL, or from avegetatively propagated plant of lettuce variety NUN 06193 LTL (or fromits progeny or from a plant having all or all but one, two or threephysiological and/or morphological characteristics which are differentfrom those of lettuce variety NUN 06193 LTL), wherein the plant part isa leaf, a harvested leaf, a part of a leaf, a head, a harvested head, apart of a head, a fruit, a harvested fruit, a part of a fruit, pollen,an ovule, a cell, a petiole, a shoot or a part thereof, a stem or a partthereof, a root or a part thereof, a root tip, a cutting, a seed, a partof a seed, seed-coat or another maternal tissue which is part of a seedgrown on lettuce variety NUN 06193 LTL, or hypocotyl, cotyledon, apistil, an anther, or a flower or a part thereof.

In another aspect, the disclosure provides a method of determining thegenotype of a plant described herein comprising detecting in the genome(e.g., a sample of nucleic acids) of the plant at least a firstpolymorphism or an allele. The skilled person is familiar with manysuitable methods of genotyping, detecting a polymorphism or detecting anallele including SNP (Single Nucleotide Polymorphism) genotyping,restriction fragment length polymorphism identification (RFLP) ofgenomic DNA, random amplified polymorphic detection (RAPD) of genomicDNA, amplified fragment length polymorphism detection (AFLP), polymerasechain reaction (PCR), DNA sequencing, allele specific oligonucleotide(ASO) probes, and hybridization to DNA microarrays or beads.Alternatively, the entire genome could be sequenced. The method may, incertain aspects, comprise detecting a plurality of polymorphisms in thegenome of the plant, for example, by obtaining a sample of nucleic acidfrom a plant and detecting in said nucleic acids a plurality ofpolymorphisms. The method may further comprise storing the results ofthe step of detecting the plurality of polymorphisms on a computerreadable medium.

The disclosure also provides for a food or feed product comprising orconsisting of a plant part described herein. Preferably, the plant partis a lettuce leaf or a lettuce head or another plant part describedherein. The food or feed product may be fresh or processed, e.g., dried,grinded, powdered, pickled, chopped, cooked, roasted, in a sauce, in asandwich, pasted, puréed or concentrated, juiced, pickled, canned,steamed, boiled, fried, blanched and/or frozen, etc.

All documents (e.g., patent publications) are herein incorporated byreference in their entirety, including the following cited references:

-   UPOV, Guidelines for the Conduct of Tests for Distinctness,    Uniformity and Stability, TG/13/11, world-wide web at upov.int under    edocs/tgdocs/en/tg013.pdf.-   US Department of Agriculture, Agricultural Marketing Service,    Objective description of Variety—Lettuce (Lactuca sativa L.), world    wide web at    ams.usda.gov/services/plant-variety-protection/pvpo-c-forms, under    lettuce.-   World Seed, Guidelines for The Handling of a Dispute on Essential    Derivation in Lettuce, world-wide web    worldseed.org/wp-content/uploads/2015/10/Guidelines_EDV_Lettuce_2004.pdf.-   Acquaah, “Principles of Plant Genetics and Breeding”, Blackwell    Publishing, 2007, ISBN-13: 978-1-4051-3646-4.-   Gonai, T., et al., “Abscisic Acid in the Thermoinhibition of Lettuce    Seed Germination and Enhancement of its Catabolism by Gibberellin”,    Journal of Experimental Botany, 2004, vol. 55(394), pp. 111-118.-   Martin, E., et al., “Identification of Markers Linked to Agronomic    Traits in Globe Artichoke”, Australian Journal of Crop Science,    2008, vol. 1(2), pp. 43-46.-   Needleman, S. B., et. al., “A General Method Applicable to the    Search for Similarities in the Amino Acid Sequence of Two Proteins”,    Journal of Molecular Biology, 1970, vol. 48(3), pp. 443-53.-   Nikolova, V., et. al., “Diploidization of Cucumber (Cucumis sativus    L.) Haploids by Colchini Treatment”, Acta Societas Botanicorum    Poloniae, 1996, vol. 65, pp. 311-317.-   Rice, P., et al., “EMBOSS: The European Molecular Biology Open    Software Suite”, Trends in Genetics, 2000, vol. 16, Issue 6. pp.    276-277.-   Teng, W., et al., “Rapid Regeneration of Lettuce from Suspension    Culture”, HortScience, 1992, vol. 27(9), pp. 1030-1032.-   Teng, W., et al., “Regenerating Lettuce from Suspension Culture in a    2-Liter Bioreactor”, HortScience, 1993, vol. 28(6), pp. 669-671.-   Vos, P., et al., “AFLP: A New Technique for DNA Fingerprinting”,    Nucleic Acids Research, 1995, vol. 23(21), pp. 4407-4414.-   Wijnker, E., et al., “Hybrid Recreation by Reverse breeding in    Arabidopsis thaliana”, Nature Protocols, 2014, vol. 9, pp. 761-772.    DOI: doi: 10.1038/nprot.2014.049-   Zhang, X., et al., “Genotypic Effects on Tissue Culture Response of    Lettuce Cotyledons”, Journal of Genetics and Breeding, 1992, vol.    46, pp. 287-290.-   EP1197137-   US2008/0222949-   US2015/0126380-   US2015/0245570-   http://anrcatalog.ucanr.edu/pdf/7215.pdf-   http://anrcatalog.ucanr.edu/pdf/7216.pdf-   https://www.ams.usda.gov/sites/default/files/media/01-Lettuce    %20ST-470-01%202015.pdf-   http://www.upov.int/edocs/tgdocs/en/tg013.pdf-   http://www.worldseed.org/wp-content/uploads/2015/10/Guidelines_EDV_Lettuce_2004.pdf-   https://www.worldatlas.com/articles/world-leaders-in-lettuce-production.html    Development of Lettuce Variety NUN 06193 LTL

The inbred variety NUN 06193 LTL was developed from an initial crossbetween lettuce lines. The female and male ancestors were crossed toproduce seeds. After the cross, progeny was self-pollinated orbackcrossed, followed by pedigree selection and line selection. Lettucevariety NUN 06193 LTL can be propagated by seeds or vegetatively, or byregeneration of a tissue culture. The seeds of lettuce variety NUN 06193LTL can be grown to produce inbred plants and parts thereof (e.g.,lettuce heads and leaves).

The Applicant concluded that lettuce variety NUN 06193 LTL is uniformand stable. This has been established through evaluation ofhorticultural characteristics. Several seed production events resultedin no observable deviation in genetic stability.

Deposit Information

A total of 2500 seeds of variety lettuce variety NUN 06193 LTL have beendeposited according to the Budapest Treaty by Nunhems B.V. on Sep. 14,2020 at the NCIMB Ltd., Ferguson Building, Craibstone Estate, Bucksburn,Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit has been assignedNCIMB 43665. A deposit of lettuce variety NUN 06193 LTL is alsomaintained at Nunhems B.V.

Access to the deposits will be available during the pendency of thisapplication to persons determined by the Director of the U.S. PatentOffice to be entitled thereto upon request. Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availabilityto the public of the deposited material will be irrevocably removed uponthe granting of the patent. The deposit will be maintained for a periodof 30 years, or 5 years after the most recent request, or for theenforceable life of the patent whichever is longer and will be replacedif it ever becomes nonviable during that period. Applicant does notwaive any rights granted under this patent on this application or underthe Plant Variety Protection Act (7 U.S.C. § 2321 et seq.).

Characteristics of Lettuce Variety NUN 06193 LTL

The most similar variety to lettuce variety NUN 06193 LTL is referred toas NUN 06109 LTL, a variety from Nunhems B.V., with the commercial nameCopious.

In Tables 1 and 2, a comparison between lettuce variety NUN 06193 LTLand Reference Variety (NUN 06109 LTL) is shown based on a trial in theUSA during the trial season 2018. Trial location: Salinas, Calif.;Harvesting date: May 5, 2018. In Table 4, the distinguishingcharacteristics between lettuce variety NUN 06193 LTL and the ReferenceVariety (NUN 06109 LTL) are shown. One replication of 30 plants pervariety, from which at least 15 plants or plant parts were randomlyselected and were used to measure characteristics. For numericalcharacteristics, averages were calculated. For non-numericalcharacteristics, the type/degree were determined.

In Table 3, the UPOV descriptors of lettuce variety NUN 06193 LTL areshown. For numerical characteristics, averages were calculated. Fornon-numerical characteristics, the type/degree were determined.

In one aspect, the disclosure provides a plant having the physiologicaland morphological characteristics of lettuce variety NUN 06193 LTL aspresented in Tables 1, 2, and 3.

TABLE 1 Objective Description of NUN 06193 LTL and Reference Variety(NUN 06109 LTL)- (USDA descriptors); where quantitative values arementioned these are statistically significantly different betweenlettuce variety NUN 06193 LTL and the Reference Variety using an ANOVATukey test. NUN 06109 LTL USDA Characteristics NUN 06193 LTL (Copious)Plant type 1 = Cutting/Leaf; 02 = Butterhead; 03 = Cos or Romaine Cos orRomaine Bibb; 04 = Cos or Romaine; 05 = Great Lakes Group; 06 = VanguardGroup; 07 = Salinas Group; 08 = Eastern (Ithaca) Group; 09 = Stem; 10 =Latin; 11 = Other (Specify) Mature leaves (harvest-mature outer leaves)Margin: Incision depth (deepest penetration of the Absent/ShallowAbsent/Shallow margin): 1 = Absent/Shallow (Dark Green Boston); 2 =Moderate (Vanguard); 3 = Deep (Great Lakes 659) Incision density (onmargin on apical part): Sparse Sparse 3 = Sparse; 5 = Medium; 7 = Dense;9 = Very Dense Indentation (finest divisions of the margin): EntireCrenate 1 = Entire; 2 = Shallowly Dentate (Great Lake 65); 3 = DeeplyDentate (Great Lake 659); 4 = Crenate (Vanguard); 5 = Other (Specify)Undulations of the apical margin: Absent Moderate 1 = Absent/Slight(Dark Green Boston); 2 = Moderate (Vanguard); 3 = Strong (Great Lakes659) Green color: Medium green Dark green 1 = Very Light Green (Bibb); 2= Light (RHS 146A) (RHS 137B) Green (Minetto); 3 = Medium Green (GreatLakes); 4 = Dark Green (Vanguard); 5 = Very Dark Green; 6 = Other(Specify) Size: Small Medium 1 = Small; 2 = Medium; 3 = LargeGlossiness: Dull Moderate 1 = Dull (Vanguard); 2 = Moderate (Salinas); 3= Glossy (Great Lakes) Blistering: Absent Moderate 1 = Absent/Slight(Salinas); 2 = Moderate (Vanguard); 3 = Strong (Prize Head) Leafthickness: Thin Intermediate 1 = Thin; 2 = Intermediate; 3 = ThickTrichomes: Absent (smooth) Absent (smooth) 1 = Absent (Smooth); 2 =Present (Spiny) Plant Spread of frame leaves (cm) 19.95 23.29 Headshape: Elongate Elongate 1 = Flattened; 2 = Slightly Flattened; 3 =Spherical; 4 = Elongate, 5 = Non-heading; 6 = Other (Specify) Head sizeclass: Small Medium 1 = Small; 2 = Medium; 3 = Large Head firmness:Loose Loose 1 = Loose; 2 = Moderate; 3 = Firm; 4 = Very Firm AdaptationPrimary regions of adaptation: 0 = Not Tested; 1 = Not Adapted; 2 =Adapted Southwest (CA and/or AZ desert) Adapted Adapted West CoastAdapted Adapted Northeast Not tested Not tested North Central Not testedNot tested Southeast Not tested Not tested Other Season: Spring WestCoast West Coast Summer West Coast West Coast Fall West Coast West CoastWinter Southwest/West Southwest/West Coast Coast

TABLE 2 Objective Description of Lettuce Variety NUN 06193 LTL andReference Variety (NUN 06109 LTL)-(Non-USDA descriptors); wherequantitative values are mentioned these are statistically significantlydifferent between Lettuce Variety NUN 06193 LTL and the ReferenceVariety using an ANOVA Tukey test. NUN 06109 LTL Non-USDACharacteristics NUN 06193 LTL (Copious) Mature leaf length, mm 145.5142.37 Mature leaf width, mm 69.98 117.03 Length/width index of 20.7912.17 mature leaf, mm Plant height, cm 14.9 13.69

TABLE 3 UPOV Characteristics of Lettuce Variety NUN 06193 LTLCharacteristics NUN 06193 LTL Seed color: White 1 white/2 yellow/3brown/4 black Seedling: Anthocyanin color: 1 absent/9 present AbsentSize of cotyledon (fully developed): 3 Medium small/5 medium/7 largeShape of cotyledon: 3 narrow elliptic/5 Narrow elliptic mediumelliptic/7 broad elliptic Plant type: Type: 1 butterhead/2 crisp/3 cos/4Cos “grasse”/5 cutting/6 stem Type: 1 butterhead/2 Batavia/3 Romaine/4Romaine Leaf Degree of overlapping of upper part of Absent or weakleaves: 1 absent or weak/2 medium/3 strong (Only varieties with plantwith absent or Many weak degree of overlapping of upper part of leaves)Plant: Number of leaves: 3 few/5 medium/7 many Leaf: Attitude: 1 erect/3semi-erect/5 Semi-erect prostate Leaf: Number of divisions: 1 absent orvery Absent or very few few/3 few/5 medium/7 many/9 very many (Onlyvarieties with absent or very few leaf Narrow elliptic divisions) Leaf:Shape: 1 triangular/2 lanceolate/3 medium oblate/4 narrow oblate/5circular/6 broad elliptic/7 medium elliptic/8 narrow elliptic/9 linear/10 broad obrullate/11 obovate/12 oblanceolate (Only varieties withabsent or very few leaf Rounded divisions) Leaf: Shape of apex: 1acute/2 obtuse/3 rounded/4 obcordate (Only varieties with absent or veryfew leaf Concave divisions) Leaf: Shape of longitudinal section: 1concave/2 flat/3 convex Leaf: Anthocyanin coloration: 1 absent or Absentvery weak/3 weak/5 medium/7 strong/9 very strong Leaf: Undulation ofmargin: 1 absent or Absent very weak/3 weak/5 medium/7 strong/9 verystrong Leaf: Depth of incisions of margin: 1 absent Absent or veryshallow/3 shallow/5 medium/7 deep/9 very deep Leaf: Venation: 1 notflabellate/2 semi- Not flabellate flabellate/3 flabellate Axillarysprouting: 1 absent or weak/2 Absent or weak medium/3 strong Boltingstem (fasciation): 1 absent or weak/ Medium to strong 3 weak/5 medium/7strong/9 very strong Leaf blade: division: 1 entire/2 lobed/3 Entiredivided Plant: Head formation: 1 no head/3 weak/5 No head medium/7strong/9 very strong Leaf: attitude at harvest maturity (outerSemi-erect leaves from head lettuce or adult leaves from cutting andstem lettuce) 1 erect/ 3 semi-erect/5 horizontal Profile of surface ofouter leaves: 3 Plane concave/5 plane/7 convex Leaf: Shape: 1 narrowelliptic/2 medium Narrow elliptic elliptic/3 broad elliptic/4 circular/5transverse broad elliptic/6 transverse narrow elliptic/7 obovate/8 broadobtrullate/9 triangular Leaf: Shape of tip: 1 acute/2 obtuse/3 Roundedrounded Leaf: Intensity of color of outer leaves: 1 Medium very light/3light/5 medium/7 dark/9 very dark Leaf: Anthocyanin coloration: 1 absentor Absent very weak/very weak to weak/3 weak/4 weak to medium/5 medium/6medium to strong/7 strong/8 strong to very strong/9 very strong Leaf:Glossiness of upper side: 1 absent or Very weak to weak very weak/3weak/5 medium/7 strong/9 very strong Leaf blade: Degree of undulation ofmargin: Absent 1 absent or very weak/3 weak/5 medium/7 strong/9 verystrong Time of beginning of bolting under long day Late conditions: 1very early/3 early/5 medium/ 7 late/9 very late Resistance to DownyMildew (Bremia Present lactucae) Isolate Bl: 16: 0 not tested/1 absent/9present Resistance to Downy Mildew (Bremia Present lactucae) Isolate Bl:17: 0 not tested/1 absent/9 present Resistance to Downy Mildew (BremiaPresent lactucae) Isolate Bl: 18: 0 not tested/1 absent/9 presentResistance to Downy Mildew (Bremia Present lactucae) Isolate Bl: 20: 0not tested/1 absent/9 present Resistance to Downy Mildew (Bremia Presentlactucae) Isolate Bl: 21: 0 not tested/1 absent/9 present Resistance toDowny Mildew (Bremia Present lactucae) Isolate Bl: 22: 0 not tested/1absent/9 present Resistance to Downy Mildew (Bremia Present lactucae)Isolate Bl: 23: 0 not tested/1 absent/9 present Resistance to DownyMildew (Bremia Present lactucae) Isolate Bl: 24: 0 not tested/1 absent/9present Resistance to Downy Mildew (Bremia Present lactucae) Isolate Bl:25: 0 not tested/1 absent/9 present Resistance to Downy Mildew (BremiaPresent lactucae) Isolate Bl: 26: 0 not tested/1 absent/9 presentResistance to Downy Mildew (Bremia Present lactucae) Isolate Bl: 27: 0not tested/1 absent/9 present Resistance to Downy Mildew (Bremia Presentlactucae) Isolate Bl: 28: 0 not tested/1 absent/9 present Resistance toDowny Mildew (Bremia Present lactucae) Isolate Bl: 31: 0 not tested/1absent/9 present Resistance to Downy Mildew (Bremia Present lactucae)Isolate Bl: 32: 0 not tested/1 absent/9 present Resistance to LettuceMosaic Virus (LMV) Present Strain Ls 1: 0 not tested/1 absent/9 presentOther resistances: Corky Root (Rhizomonas subefaciens)

TABLE 4 Distinguishing Characteristics between Lettuce Variety NUN 06193LTL and Reference Variety NUN 06109 LTL (based on USDA trial) NUN 06109LTL Characteristics NUN 06193 LTL (Copious) Plant height, cm 14.9  13.69Plant spread, cm 19.95 23.29 Mature leaf width, mm 69.98 117.03 Length/width index of mature leaf, 20.79 12.17 mm Mature leafindentation Entire Crenate Mature leaf undulations of the AbsentModerate apical margins Mature leaf color Medium green Dark green (RHS146A) (RHS 137B) Mature leaf size Small Medium Mature leaf glossinessDull Moderate Mature leaf blistering Absent Moderate Mature leafthickness Thin Intermediate Plant head size class Small Medium

The invention claimed is:
 1. A plant, plant part or seed of lettucevariety NUN 06193 LTL, wherein a representative sample of seed of saidlettuce variety is deposited under Accession Number NCIMB
 43665. 2. Theplant part of claim 1, wherein said plant part is a leaf, a head, apollen, a stem, an ovule, a fruit, a scion, a rootstock, a cutting, aflower, or a cell.
 3. A seed grown from the plant of claim
 1. 4. Alettuce plant or part thereof grown from the seed of claim
 1. 5. Alettuce plant, or a part thereof having all the physiological andmorphological characteristics of the plant of claim
 1. 6. A tissue orcell culture comprising regenerable cells of the plant of claim
 1. 7.The tissue or cell culture according to claim 6, comprising cells orprotoplasts derived from a plant part, wherein the plant part is anembryo, a meristem, a cotyledon, a hypocotyl, a pollen, a leaf, ananther, a root, a root tip, a pistil, a petiole, a flower, a fruit, aseed, a stem, or a stalk.
 8. A lettuce plant regenerated from the tissueor cell culture of claim 7, wherein the plant has all of thephysiological and morphological characteristics of the plant of lettucevariety NUN 06193, when the numerical characteristics are determined atthe 5% significance level for plants grown under the same environmentalconditions, and wherein a representative sample of seed of said varietyis deposited under Accession Number NCIMB
 43665. 9. A method ofproducing the plant of claim 1 or a part thereof, said method comprisingvegetative propagation of lettuce variety NUN 06193 LTL, wherein arepresentative sample of seed of said variety is deposited underAccession Number NCIMB
 43665. 10. The method of claim 9, wherein saidvegetative propagation comprises regenerating a whole plant from a partof lettuce variety NUN 06193 LTL, wherein a representative sample ofseed of said variety is deposited under Accession Number NCIMB 43665.11. The method of claim 9, wherein said part is a cutting, a cellculture or a tissue culture.
 12. A vegetative propagated plant of claim1, or a part thereof, wherein the vegetative propagated plant has all ofthe physiological and morphological characteristics of the plant oflettuce variety NUN 06193 LTL, when the numerical characteristics aredetermined at the 5% significance level for plants grown under the sameenvironmental conditions, and wherein a representative sample of seed ofsaid lettuce variety is deposited under Accession Number NCIMB 43665.13. A method of producing a lettuce plant, said method comprisingcrossing the plant of claim 1 with a second lettuce plant at least once,and selecting a progeny lettuce plant from said crossing and allowingthe progeny lettuce plant to form seed.
 14. A first generation progenyplant of the lettuce plant of claim 1 obtained by crossing the plant oflettuce variety NUN 06193 LTL with itself or another lettuce plant,wherein said progeny has all the morphological and physiologicalcharacteristics of lettuce variety NUN 06193 LTL, and wherein arepresentative sample of seed of said lettuce variety is deposited underAccession Number NCIMB
 43665. 15. A lettuce plant having onephysiological or morphological characteristic which is different fromthose of the plant of claim 1, and which otherwise has all thephysiological and morphological characteristics of lettuce variety NUN06193 LTL, when the numerical characteristics are determined at the 5%significance level for plants grown under the same environmentalconditions.
 16. A plant of lettuce variety NUN 06193 LTL furthercomprising a transgene conferring a desired trait and otherwise havingall of the morphological and physiological characteristics of the plantof claim 1, when grown under the same environmental conditions, whereina representative sample of seed of said lettuce variety is depositedunder Accession Number NCIMB 43665, and wherein the desired trait isyield, storage properties, male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, environmental stresstolerance, modified carbohydrate metabolism or modified proteinmetabolism.
 17. A method of making doubled haploid cells of lettucevariety NUN 06193 LTL, said method comprising making double haploidcells from the plant or seed of lettuce variety NUN 06193 LTL, wherein arepresentative sample of seed of lettuce variety NUN 06193 LTL isdeposited under Accession Number NCIMB
 43665. 18. A container comprisingthe plant or plant part of claim
 1. 19. A food, or a feed product, or aprocessed product comprising the plant part of claim 2, wherein theplant part is a leaf or part thereof.
 20. A method of producing amodified lettuce plant, said method comprising mutating a lettuce plantor plant part of lettuce variety NUN 06193 LTL, wherein a representativesample of seed of said lettuce variety is deposited under AccessionNumber NCIMB
 43665. 21. A container comprising the seed of claim
 1. 22.A plant of lettuce variety NUN 06193 LTL further comprising a singlelocus conversion, wherein said plant otherwise has all of thephysiological and morphological characteristics of the plant of claim 1when grown under the same environmental conditions, wherein arepresentative sample of seed of said lettuce variety is deposited underAccession Number NCIMB 43665, and wherein the single locus conversionconfers yield, storage properties, color, male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,environmental stress tolerance, modified carbohydrate metabolism, ormodified protein metabolism.
 23. A method of producing a lettuce head ora lettuce leaf, comprising growing the plant, plant part or seed ofclaim 1 until it develops at least leaf or head, and collecting the leafor head.